Ebook Info.

TextBook of Fish Cell Culture

Goswami, M

Year: 2022
eISBN: 9789356442498
Language: ENGLISH

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PART-I 
1. Introduction 2. Biology of Tissue and Cell Culture 3. Development and Characterization of Cell Line 4. Maintenance and Cryopreservation of Cell Line 5. Development and Applications of Stem Cell Culture 6. Three Dimensional Cell Culture and Tissue Engineering 7. Cell Line Repository 8. Contaminants in Cell Culture 9. Good Laboratory Practices 10. Media and Reagents 11. Design and Equipment for the Cell Culture Laboratory

PART-II 
12. Sterilization of Glassware and Culture Media13. Procedure for Development of Cell Culture and Characterization14. Detailed Protocol for the Preparation of Media 15. Detailed Protocol for Establishment of Primary Culture 16. Detailed Protocol for Cell Counting using Haemocytometer 17. Detailed Protocol for DNA Isolation from Fish Cell Line 18. Detailed Protocol for Cell Line 
19. Detailed Protocol for Preparation of Chromosomes from Cell Culture 20. Detailed Protocol for Transfection of Cell Line with GFP Reporter Gene 21. Detailed Protocol for Protein Expression Signature (PES) 22. Detailed Protocol for Cytotoxicity Assessment 23. Detailed Protocol for Genotoxicity Assessment 24. Monographs of Fish Cell Line 25. List of Fish Cell Lines Developed by our Research Group in India